The DNA-DNA relatedness values between strain THG 07 T and its closest phylogenetic neighbors were below 26.9%. The major menaquinone, MK-7, and major fatty acids, iso-C 15:0 and C 16:1 ω7c and/or ω6 c, supported the affiliation of strain THG 07 T to the genus Sphingobacterium. The G+C content of the genomic DNA was 40.6 mol%. On the basis of 16S rRNA gene sequence similarity, strain THG 07 T was shown to belong to the family Sphingobacteriaceae and was related to Sphingobacterium canadense CR11 T (98.7%), S. Strain THG-T17 T displayed β-glucosidase activity that was responsible for its ability to transform ginsenoside Rb 1 (one of the dominant ginsenosides of ginseng) to compound C-K. Strain THG 07 T grew optimally at 25−30☌ and at pH 6.5−7.0 and in the absence of NaCl on nutrient agar. #0 Foam::error::printStack(Foam::Ostream&) at ?:? #1 Foam::error::abort() at ?:? #2 Foam::heRhoThermo >, Foam::sensibleEnthalpy>::calculate() at ?:? #3 Foam::heRhoThermo >, Foam::sensibleEnthalpy>::correct() at ?:? #4 ? at ?:? #5 _libc_start_main in "/lib/x86_64-linux-gnu/libc.so.A Gram-staining-negative, aerobic, non-motile, non-spore-forming, and rod-shaped bacterium designated THG 07 T was isolated from the soil of a ginseng field of Pocheon in South Korea, and its taxonomic position was investigated by using a polyphasic study. > FOAM FATAL ERROR: Maximum number of iterations exceededįrom function Foam::scalar Foam::species::thermo::T(Foam::scalar, Foam::scalar, Foam::scalar, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar) const) const in file /home/ubuntu/OpenFOAM/OpenFOAM-4.1/src/thermophysicalModels/specie/lnInclude/thermoI.H at line 66. I have some problem with chtMultiRegionSimpleFoam Then you can decide which sites need to be checked in your original chromatograms to decide whether or not to edit a sequence.īioEdit will also produce a consensus sequence with the pull-down menu item Alignment:Create Consensus Sequence, but it may be better to edit incorrect base calls first. Once all of the sequences are aligned, you can easily highlight sites where not all of the sequences are identical using the pulldown menu for Alignment:Plot Identities to first sequence with a dot. You can use CLUSTAL which is built in to BioEdit for this, under the Accessory Application pull-down menu. This will "invert" your reverse sequence(s) so it (they) should now align with the forward sequence(s). If this is true, you can easily convert the reverse sequence(s) to forward by selecting the reverse sequence(s) and then using the pull-down menu for Sequence:Nuleic Acid:Reverse Complement It sounds like you already have the sequences of a PCR product, sequenced from the forward and reverse primers, in BioEdit.
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